HealthDay News — Pollen-derived adenosine is an important element in ragweed pollen-induced allergic airway inflammation, study findings indicate.
“Ragweed (Ambrosia artemisiifolia) is a strong elicitor of allergic airway inflammation with worldwide increasing prevalence,” noted Maria Wimmer, PhD, of the Technische Universität München in Germany, and colleagues in Allergy.
To identify critical factors for allergenicity of ragweed pollen in a physiological model of allergic airway inflammation, the investigators instilled aqueous ragweed pollen extract, the low molecular weight fraction, or major allergen Amb a 1 intranasally on one to 11 consecutive days.
Allergic airway inflammation was assessed. In order to examine the role of pollen-derived adenosine in ragweed-induced allergy, it was removed from the extract enzymatically. The authors also examined migration of human neutrophils and eosinophils toward supernatants of ragweed-stimulated bronchial epithelial cells.
Specific immunoglobulin G1, pulmonary infiltration with inflammatory cells, a Th2-associated cytokine signature in pulmonary tissues, and impaired lung function were induced by instillation of ragweed pollen extract, but not of the major allergen or the low molecular weight fraction.
Ragweed-induced allergic lung inflammation was aggravated by adenosine. If adenosine was present, human neutrophils and eosinophils migrated toward supernatants of bronchial epithelial cells stimulated with ragweed extract in vitro.
“Pollen-derived adenosine is a critical factor in ragweed-pollen induced allergic airway inflammation,” concluded the scientists.
“Future studies aim at therapeutic strategies to control these allergen-independent pathways.”