HealthDay News — A rapid and sensitive assay requiring no special equipment can detect Chlamydia trachomatis directly from urine samples with high accuracy, study findings indicate.
Although several point-of-care tests have been developed for C. trachomatis diagnostics and many are fast and specific, they lack the required sensitivity for large-scale application.
Katrin Krõlov, from the University of Tartu in Estonia, and colleagues describe a novel assay to detect C. trachomatis in urine samples using recombinase polymerase amplification of a highly conserved target within the CDS2 gene in the Journal of Molecular Diagnostics.
The accuracy of the novel assay was tested on urine samples from 70 patients attending a sexual health clinic and results were compared with the Roche Cobas Amplicor C. trachomatis assay.
Initial analysis of the novel assay showed specificity of 100% (95% CI: 92%–100%) and an 83% sensitivity (95% CI, 51%–97%), the researchers found. The urine test had a minimum detection limit of five to 12 pathogens and could detect pathogen directly in urine within 20 minutes.
“The whole procedure is fairly simple and does not require specific machinery, making it potentially applicable in point-of-care settings,” Krolov and colleagues concluded.